Ketohexokinase inhibition improves NASH by reducing fructose-induced steatosis and fibrogenesis.
JHEP Rep
; 3(2): 100217, 2021 Apr.
Article
in En
| MEDLINE
| ID: mdl-33490936
ABSTRACT
BACKGROUND & AIMS:
Increasing evidence highlights dietary fructose as a major driver of non-alcoholic fatty liver disease (NAFLD) pathogenesis, the majority of which is cleared on first pass through the hepatic circulation by enzymatic phosphorylation to fructose-1-phosphate via the ketohexokinase (KHK) enzyme. Without a current approved therapy, disease management emphasises lifestyle interventions, but few patients adhere to such strategies. New targeted therapies are urgently required.METHODS:
We have used a unique combination of human liver specimens, a murine dietary model of NAFLD and human multicellular co-culture systems to understand the hepatocellular consequences of fructose administration. We have also performed a detailed nuclear magnetic resonance-based metabolic tracing of the fate of isotopically labelled fructose upon administration to the human liver.RESULTS:
Expression of KHK isoforms is found in multiple human hepatic cell types, although hepatocyte expression predominates. KHK knockout mice show a reduction in serum transaminase, reduced steatosis and altered fibrogenic response on an Amylin diet. Human co-cultures exposed to fructose exhibit steatosis and activation of lipogenic and fibrogenic gene expression, which were reduced by pharmacological inhibition of KHK activity. Analysis of human livers exposed to 13C-labelled fructose confirmed that steatosis, and associated effects, resulted from the accumulation of lipogenic precursors (such as glycerol) and enhanced glycolytic activity. All of these were dose-dependently reduced by administration of a KHK inhibitor.CONCLUSIONS:
We have provided preclinical evidence using human livers to support the use of KHK inhibition to improve steatosis, fibrosis, and inflammation in the context of NAFLD. LAYSUMMARY:
We have used a mouse model, human cells, and liver tissue to test how exposure to fructose can cause the liver to store excess fat and become damaged and scarred. We have then inhibited a key enzyme within the liver that is responsible for fructose metabolism. Our findings show that inhibition of fructose metabolism reduces liver injury and fibrosis in mouse and human livers and thus this may represent a potential route for treating patients with fatty liver disease in the future.
ALD, alcohol-related cirrhosis; ALT, alanine transaminase; APRI, AST to Platelet Ratio Index; AST, aspartate transaminase; BEC, biliary epithelial cells; BSA, bovine serum albumin; CT, computed tomography; DNL, de novo lipogenesis; FIB4, fibrosis-4; Fibrosis; Fructose; G/F, glucose/fructose; HSCs, hepatic stellate cells; HSECs, hepatic sinusoidal endothelial cells; HSQC, heteronuclear single quantum coherence; IGF, insulin-like growth factor; KHK, ketohexokinase; KO, knockout; LGLI, low glucose and insulin; Metabolism; NAFLD; NAFLD, non-alcoholic fatty liver disease; NASH; NASH, non-alcoholic steatohepatitis; NPCs, non-parenchymal cells; PBC, primary biliary cholangitis; PDGF, platelet-derived growth factor; PSC, primary sclerosing cholangitis; TG, triglyceride; TGFB, transforming growth factor beta; TIMP-1, Tissue Inhibitor of Matrix metalloproteinase-1; Treatment; WT, wild-type; aLMF, activated liver myofibroblasts
Full text:
1
Database:
MEDLINE
Type of study:
Prognostic_studies
Language:
En
Journal:
JHEP Rep
Year:
2021
Type:
Article
Affiliation country:
United kingdom