Tunable expression rate control of a growth-decoupled T7 expression system by L-arabinose only.
Microb Cell Fact
; 20(1): 27, 2021 Feb 01.
Article
in En
| MEDLINE
| ID: mdl-33522916
ABSTRACT
BACKGROUND:
Precise regulation of gene expression is of utmost importance for the production of complex membrane proteins (MP), enzymes or other proteins toxic to the host cell. In this article we show that genes under control of a normally Isopropyl ß-D-1-thiogalactopyranoside (IPTG)-inducible PT7-lacO promoter can be induced solely with L-arabinose in a newly constructed Escherichia coli expression host BL21-AIRESULTS:
Here, we show that BL21-AICONCLUSIONS:
In all cases tested, BL21-AIKey words
Full text:
1
Database:
MEDLINE
Main subject:
Arabinose
/
Gene Expression Regulation, Bacterial
/
Bacteriophage T7
/
Escherichia coli
Language:
En
Journal:
Microb Cell Fact
Journal subject:
BIOTECNOLOGIA
/
MICROBIOLOGIA
Year:
2021
Type:
Article
Affiliation country:
Austria