Using Mass Cytometry to Analyze the Tumor-Infiltrating Lymphocytes in Human Melanoma.
Methods Mol Biol
; 2265: 543-555, 2021.
Article
in En
| MEDLINE
| ID: mdl-33704739
ABSTRACT
Here we describe the application of mass cytometry to analyze tumor-infiltrating lymphocytes in human melanoma. Mass cytometry is the coupling of flow cytometry and mass spectrometry, which allows for the simultaneous measurement of 40+ cell parameters on a per cell basis. Heavy metal-labeled antibodies can bind to proteins (CD markers, transcription factors, cytokines) on the cell surface and in the cytoplasm/nucleus. As labeled cells pass through the CyTOF, the instrument detects the heavy metals. Combining these signals allows description of melanoma tumor-infiltrating lymphocytes at a greater depth than alternative phenotyping strategies and enables detailed analyses of a variety of cellular parameters, including immune cell lineage, activation status, and functional polarization.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Mass Spectrometry
/
Lymphocytes, Tumor-Infiltrating
/
Single-Cell Analysis
/
Flow Cytometry
/
Melanoma
Limits:
Humans
Language:
En
Journal:
Methods Mol Biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2021
Type:
Article
Affiliation country:
Australia