Buffers, Especially the Good Kind.
Biochemistry
; 60(46): 3436-3440, 2021 11 23.
Article
in En
| MEDLINE
| ID: mdl-33939915
ABSTRACT
Fifty-five years ago, Norman Good and colleagues authored a paper that fundamentally advanced wet biochemistry [Good, N. E., Winget, G. D., Winter, W., Connolly, T. N., Izawa, S., and Singh, R. M. M. (1966) Hydrogen ion buffers for biological research. Biochemistry 5, 467-477] and in doing so has amassed more than 2500 citations. They laid out the properties required for useful, biochemically relevant hydrogen-ion buffers and then synthesized and tested 10 of them. Soon after, these buffers became commercially available. Since then, most of us never gave them a second thought. We just use them. Here, I discuss some of the background regarding the genesis of "Good's buffers", make a few (disparaging) observations about the non-Good's buffer, Tris, and suggest that we synthesize new buffers by combining the ideas of Good et al. with results from the past 60 years of protein chemistry.
Full text:
1
Database:
MEDLINE
Main subject:
Biochemistry
/
Buffers
Language:
En
Journal:
Biochemistry
Year:
2021
Type:
Article
Affiliation country:
United States