Protocol for the derivation, culturing, and differentiation of human iPS-cell-derived neuroepithelial stem cells to study neural differentiation in vitro.
STAR Protoc
; 2(2): 100528, 2021 06 18.
Article
in En
| MEDLINE
| ID: mdl-34027486
ABSTRACT
Here, we present a revised protocol to derive neuroepithelial stem (NES) cells from human induced pluripotent stem cells. NES cells can be further differentiated into a culture of neurons (90%) and glia (10%). We describe how to derive and maintain NES cells in culture and how to differentiate them. In addition, we show the potential use of NES cells to study the role of reactive oxygen species in neuronal differentiation and a guideline for NES cell transfection. For complete details on the use and execution of this protocol, please refer to Calvo-Garrido et al. (2019); Falk et al. (2012).
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Cell Differentiation
/
Cell Culture Techniques
/
Neuroepithelial Cells
/
Induced Pluripotent Stem Cells
Limits:
Humans
Language:
En
Journal:
STAR Protoc
Year:
2021
Type:
Article
Affiliation country:
Sweden