Nonalcoholic fatty liver disease stratification by liver lipidomics.
J Lipid Res
; 62: 100104, 2021.
Article
in En
| MEDLINE
| ID: mdl-34384788
ABSTRACT
Nonalcoholic fatty liver disease (NAFLD) is a common metabolic dysfunction leading to hepatic steatosis. However, NAFLD's global impact on the liver lipidome is poorly understood. Using high-resolution shotgun mass spectrometry, we quantified the molar abundance of 316 species from 22 major lipid classes in liver biopsies of 365 patients, including nonsteatotic patients with normal or excessive weight, patients diagnosed with NAFL (nonalcoholic fatty liver) or NASH (nonalcoholic steatohepatitis), and patients bearing common mutations of NAFLD-related protein factors. We confirmed the progressive accumulation of di- and triacylglycerols and cholesteryl esters in the liver of NAFL and NASH patients, while the bulk composition of glycerophospho- and sphingolipids remained unchanged. Further stratification by biclustering analysis identified sphingomyelin species comprising n242 fatty acid moieties as membrane lipid markers of NAFLD. Normalized relative abundance of sphingomyelins SM 433;2 and SM 431;2 containing n242 and n240 fatty acid moieties, respectively, showed opposite trends during NAFLD progression and distinguished NAFL and NASH lipidomes from the lipidome of nonsteatotic livers. Together with several glycerophospholipids containing a C226 fatty acid moiety, these lipids serve as markers of early and advanced stages of NAFL.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Non-alcoholic Fatty Liver Disease
/
Lipidomics
/
Liver
Type of study:
Prognostic_studies
Limits:
Adolescent
/
Adult
/
Aged
/
Aged80
/
Female
/
Humans
/
Male
/
Middle aged
Language:
En
Journal:
J Lipid Res
Year:
2021
Type:
Article
Affiliation country:
Germany