Tumor Necrosis Factor Alpha-Induced Interleukin-1 Alpha Synthesis and Cell Death Is Increased in Mouse Epithelial Cells Infected With Chlamydia muridarum.
J Infect Dis
; 224(12 Suppl 2): S47-S55, 2021 08 16.
Article
in En
| MEDLINE
| ID: mdl-34396406
ABSTRACT
Chlamydia trachomatis-genital infection in women can be modeled in mice using Chlamydia muridarum. Using this model, it has been shown that the cytokines tumor necrosis factor (TNF)α and interleukin (IL)-1α lead to irreversible tissue damage in the oviducts. In this study, we investigated the contribution of TNFα on IL-1α synthesis in infected epithelial cells. We show that C muridarum infection enhanced TNFα-induced IL-1α expression and release in a mouse epithelial cell line. In addition to IL-1α, several TNFα-induced inflammatory genes were also highly induced, and infection enhanced TNF-induced cell death. In the mouse model of genital infection, oviducts from mice lacking the TNFα receptor displayed minimal staining for IL-1α compared with wild-type oviducts. Our results suggest TNFα and IL-1α enhance each other's downstream effects resulting in a hyperinflammatory response to chlamydial infection. We propose that biologics targeting TNF-induced IL-1α synthesis could be used to mitigate tissue damage during chlamydial infection.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Chlamydia Infections
/
Tumor Necrosis Factor-alpha
/
Cell Death
/
Chlamydia muridarum
/
Interleukin-1alpha
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
J Infect Dis
Year:
2021
Type:
Article
Affiliation country:
United States