Evaluation of the Binding Kinetics of RHEB with mTORC1 by In-Cell and In Vitro Assays.

Shams, Raef; Ito, Yoshihiro; Miyatake, Hideyuki

Shams, Raef; Ito, Yoshihiro; Miyatake, Hideyuki.

Affiliation

Shams R; Emergent Bioengineering Materials Research Team, RIKEN Center for Emergent Matter Science, RIKEN, Wako 351-0198, Saitama, Japan.
Ito Y; Department of Life Science, Graduate School of Science and Engineering, Saitama University, Saitama City 338-8570, Saitama, Japan.
Miyatake H; Emergent Bioengineering Materials Research Team, RIKEN Center for Emergent Matter Science, RIKEN, Wako 351-0198, Saitama, Japan.

Int J Mol Sci ; 22(16)2021 Aug 16.

Article in En | MEDLINE | ID: mdl-34445471

Subject(s)

Mechanistic Target of Rapamycin Complex 1/metabolism; Protein Interaction Domains and Motifs; Ras Homolog Enriched in Brain Protein/metabolism; Binding Sites; HEK293 Cells; Humans; In Vitro Techniques; Kinetics; Mechanistic Target of Rapamycin Complex 1/genetics; Phosphorylation; Ras Homolog Enriched in Brain Protein/genetics

Key words

G-Protein; RHEB; allosteric activation; binding kinetics; kinase domain; mTORC1

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Full text: 1 Database: MEDLINE Main subject: Protein Interaction Domains and Motifs / Mechanistic Target of Rapamycin Complex 1 / Ras Homolog Enriched in Brain Protein Limits: Humans Language: En Journal: Int J Mol Sci Year: 2021 Type: Article Affiliation country: Japan

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