Preparation of Site-Specifically Spin-Labeled RNA by in Vitro Transcription Using an Expanded Genetic Alphabet.

Bornewasser, Lisa; Kath-Schorr, Stephanie

Bornewasser, Lisa; Kath-Schorr, Stephanie.

Affiliation

Bornewasser L; Department of Chemistry, University of Cologne, Köln, Germany.
Kath-Schorr S; Department of Chemistry, University of Cologne, Köln, Germany. skathsch@uni-koeln.de.

Methods Mol Biol ; 2439: 223-240, 2022.

Article in En | MEDLINE | ID: mdl-35226325

Subject(s)

Oligonucleotides; RNA; Electron Spin Resonance Spectroscopy/methods; Oligonucleotides/chemistry; Oligonucleotides/genetics; RNA/chemistry; RNA/genetics; Spin Labels

Key words

Electron paramagnetic resonance (EPR); Expanded genetic alphabet; In vitro transcription; Pulsed electronelectron double resonance (PELDOR); RNA; Spin labeling; Unnatural base pairs

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Full text: 1 Database: MEDLINE Main subject: Oligonucleotides / RNA Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2022 Type: Article Affiliation country: Germany

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