Loss of KAP3 decreases intercellular adhesion and impairs intracellular transport of laminin in signet ring cell carcinoma of the stomach.
Sci Rep
; 12(1): 5050, 2022 03 23.
Article
in En
| MEDLINE
| ID: mdl-35322078
ABSTRACT
Signet-ring cell carcinoma (SRCC) is a unique subtype of gastric cancer that is impaired for cell-cell adhesion. The pathogenesis of SRCC remains unclear. Here, we show that expression of kinesin-associated protein 3 (KAP3), a cargo adaptor subunit of the kinesin superfamily protein 3 (KIF3), a motor protein, is specifically decreased in SRCC of the stomach. CRISPR/Cas9-mediated gene knockout experiments indicated that loss of KAP3 impairs the formation of circumferential actomyosin cables by inactivating RhoA, leading to the weakening of cell-cell adhesion. Furthermore, in KAP3 knockout cells, post-Golgi transport of laminin, a key component of the basement membrane, was inhibited, resulting in impaired basement membrane formation. Together, these findings uncover a potential role for KAP3 in the pathogenesis of SRCC of the stomach.
Full text:
1
Database:
MEDLINE
Main subject:
Stomach Neoplasms
/
Carcinoma, Signet Ring Cell
Limits:
Humans
Language:
En
Journal:
Sci Rep
Year:
2022
Type:
Article
Affiliation country:
Japan