An optimal saponification and extraction method to determine carotenoids in avocado.
Food Chem
; 387: 132923, 2022 Sep 01.
Article
in En
| MEDLINE
| ID: mdl-35427868
ABSTRACT
Quantification of carotenoids in avocado fruit is a great challenge due to their co-extraction with high-oil concentration and the inherent nature of carotenoids to degrade and undergo cis/trans photoisomerization with prolonged extraction times and high temperatures. The study provides an optimised and validated methodology for quantification of carotenoids in the high-oil avocado matrix, with > 93% recovery of all carotenoids tested being significantly greater than previously published. Saponification with 15% KOH for 60 min was optimal for the avocado matrix. For the first time, this study identified that soap, produced during the saponification reaction, resulted in a significant reduction of carotenoid content from the avocado matrix, due to the production of micelles. A significantly higher carotenoid content (3.58 versus 2.0 mg/100 g DW) was able to be extracted after saponification with acidified phosphate buffer, instead of water as reported previously. Carotenoid profiles of five avocado cultivars were identified and quantified.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Carotenoids
/
Persea
Language:
En
Journal:
Food Chem
Year:
2022
Type:
Article