A method for the isolation of &#945;-ribazole from vitamin B<sub>12</sub>, and its enzymatic conversion to &#945;-ribazole 5'-phosphate.

Malalasekara, Lahiru; Escalante-Semerena, Jorge C

A method for the isolation of α-ribazole from vitamin B₁₂, and its enzymatic conversion to α-ribazole 5'-phosphate.

Malalasekara, Lahiru; Escalante-Semerena, Jorge C.

Affiliation

Malalasekara L; Department of Microbiology, University of Georgia, Athens, GA, United States.
Escalante-Semerena JC; Department of Microbiology, University of Georgia, Athens, GA, United States. Electronic address: jcescala@uga.edu.

Methods Enzymol ; 668: 125-136, 2022.

Article in En | MEDLINE | ID: mdl-35589191

Subject(s)

Phosphates; Vitamin B 12; Cobalt/chemistry; Cobamides/chemistry; Cobamides/metabolism; Coenzymes; Ligands; Ribonucleosides; Vitamin B 12/metabolism; Vitamins

Key words

Boronate affinity chromatography; Coenzyme B(12); Late steps of coenzyme B(12) biosynthesis; Scale-up synthesis of α-ribazole; Vitamin B(12) hydrolysis; α-Ribazole kinase; α-Ribazole-phosphate

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Full text: 1 Database: MEDLINE Main subject: Phosphates / Vitamin B 12 Language: En Journal: Methods Enzymol Year: 2022 Type: Article Affiliation country: United States

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