Knockdown of IFNAR2 reduces the inflammatory response in mouse model of type 1 diabetes.
Biochem Biophys Res Commun
; 619: 9-14, 2022 09 03.
Article
in En
| MEDLINE
| ID: mdl-35728283
ABSTRACT
BACKGROUND:
andPurpose:
To investigate the biological role of interferon α/ß receptor 2 (IFNAR2) in type 1 diabetes (T1D).METHODS:
First, IFNAR2 mRNA and protein expression levels in serum of T1D patients and healthy controls were detected by RT-qPCR and Western blot. For experimental studies, 80 male C57BL/6 mice were randomly divided into 4 groups with 20 mice in each group the control group, the T1D group, the T1D + ad-con group and the T1D + ad-si-IFNAR2 group. The T1D mouse model was generated by multiple intraperitoneal injections of small doses of streptozotocin (STZ). Body weight and blood glucose levels were measured weekly until 6 weeks. After 6 weeks, all mice were sacrificed and the levels of insulin (Ins), tumor necrosis factor α (TNF-α), interleukin 4 (IL-4), IL-6, and type I interferon γ (IFN-γ), IFNAR2 protein expression, the number of dendritic cells (DCs), and changes in islet ß cells were assessed.RESULTS:
IFNAR2 mRNA and protein expression levels in serum of T1D patients were significantly higher than those in healthy controls (P < 0.05). Furthermore, IFNAR2 protein expression, number of DCs, and IFNAR2 mRNA, blood glucose, TNF-α, and IFN-γ levels were significantly upregulated in T1D mice compared with the control group (P < 0.05), while weight, and Ins, IL-6, and IL-4 levels were decreased (P < 0.05). However, knockdown of IFNAR2 reversed these trends. There was no significant difference in markers between the T1D + ad-con group and the T1D group (P > 0.05).CONCLUSIONS:
Knockdown of IFNAR2 reduced the inflammatory response and improved islet function of T1D mice.Key words
Full text:
1
Database:
MEDLINE
Main subject:
Diabetes Mellitus, Type 1
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Biochem Biophys Res Commun
Year:
2022
Type:
Article
Affiliation country:
China