Rapid detection of an Ebola biomarker with optical microring resonators.
Cell Rep Methods
; 2(6): 100234, 2022 06 20.
Article
in En
| MEDLINE
| ID: mdl-35784644
ABSTRACT
Ebola virus (EBOV) is a highly infectious pathogen, with a case mortality rate as high as 89%. Rapid therapeutic treatments and supportive measures can drastically improve patient outcome; however, the symptoms of EBOV disease (EVD) lack specificity from other endemic diseases. Given the high mortality and significant symptom overlap, there is a critical need for sensitive, rapid diagnostics for EVD. Facile diagnosis of EVD remains a challenge. Here, we describe a rapid and sensitive diagnostic for EVD through microring resonator sensors in conjunction with a unique biomarker of EBOV infection, soluble glycoprotein (sGP). Microring resonator sensors detected sGP in under 40 min with a limit of detection (LOD) as low as 1.00 ng/mL in serum. Furthermore, we validated our assay with the detection of sGP in serum from EBOV-infected non-human primates. Our results demonstrate the utility of a high-sensitivity diagnostic platform for detection of sGP for diagnosis of EVD.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Hemorrhagic Fever, Ebola
/
Ebolavirus
Type of study:
Diagnostic_studies
Limits:
Animals
/
Humans
Language:
En
Journal:
Cell Rep Methods
Year:
2022
Type:
Article
Affiliation country:
United States