Ethylenediaminetetraacetic acid (EDTA) enhances cAMP production in human TDAG8-expressing cells.
Biochem Biophys Res Commun
; 626: 15-20, 2022 10 20.
Article
in En
| MEDLINE
| ID: mdl-35964552
ABSTRACT
Ethylenediaminetetraacetic acid (EDTA) is a chelating agent that binds tightly to metal ions. We found that cAMP response element (CRE)-driven promoter activity by protons was enhanced by EDTA in human T-cell death-associated gene 8 (TDAG8)-overexpressed HEK293T cells. The enhancing action by EDTA was also detected by proton-induced cAMP production that is located upstream from the CRE-driven promoter activity even at physiological proton concentration pH7.4. The proton-induced CRE-driven promoter activity was not enhanced by other chelating agents, ethylene glycol tetraacetic acid (EGTA) and sodium citrate. The enhanced CRE-driven promoter activity by EDTA was not attenuated by increasing the extracellular calcium ion concentration. These results indicate that the EDTA-enhancing action may not be due to its chelating action but might rather be another EDTA-specific effect. Enhanced cAMP production by EDTA was also detected in a human leukemia cell line HL-60, in which TDAG8 and OGR1 (ovarian cancer G-protein-coupled receptor 1) were endogenously expressed, suggesting that the medical use of EDTA would influence the physiological and pathophysiological functions of hematopoietic cells.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Protons
/
Cyclic AMP
Limits:
Humans
Language:
En
Journal:
Biochem Biophys Res Commun
Year:
2022
Type:
Article
Affiliation country:
Japan