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Comprehensive characterization of ubiquitinome of human colorectal cancer and identification of potential survival-related ubiquitination.
Zhang, Wei; Yang, Yan; Lin, Liewen; He, Jingquan; Dong, Jingjing; Yan, Bin; Cai, Wanxia; Chen, Yumei; Yin, Lianghong; Tang, Donge; Liu, Fanna; Dai, Yong.
Affiliation
  • Zhang W; The First Affiliated Hospital, Jinan University, 613 W. Huangpu Avenue, Guangzhou, Guangdong, 510632, China.
  • Yang Y; Department of Clinical Medical Research Center, Guangdong Provincial Engineering Research Center of Autoimmune Disease Precision Medicine The Second Clinical Medical College, Jinan University (Shenzhen People's Hospital), Shenzhen, Guangdong, 518020, China.
  • Lin L; The First Affiliated Hospital, Jinan University, 613 W. Huangpu Avenue, Guangzhou, Guangdong, 510632, China.
  • He J; Department of Nephrology, Institute of Nephrology and Blood Purification, The First Affiliated Hospital of Jinan University, Jinan University, Guangzhou, Guangdong, 510632, China.
  • Dong J; Department of Clinical Medical Research Center, Guangdong Provincial Engineering Research Center of Autoimmune Disease Precision Medicine The Second Clinical Medical College, Jinan University (Shenzhen People's Hospital), Shenzhen, Guangdong, 518020, China.
  • Yan B; Department of Clinical Medical Research Center, Guangdong Provincial Engineering Research Center of Autoimmune Disease Precision Medicine The Second Clinical Medical College, Jinan University (Shenzhen People's Hospital), Shenzhen, Guangdong, 518020, China.
  • Cai W; The First Affiliated Hospital, Jinan University, 613 W. Huangpu Avenue, Guangzhou, Guangdong, 510632, China.
  • Chen Y; Department of Nephrology, Institute of Nephrology and Blood Purification, The First Affiliated Hospital of Jinan University, Jinan University, Guangzhou, Guangdong, 510632, China.
  • Yin L; Department of Clinical Medical Research Center, Guangdong Provincial Engineering Research Center of Autoimmune Disease Precision Medicine The Second Clinical Medical College, Jinan University (Shenzhen People's Hospital), Shenzhen, Guangdong, 518020, China.
  • Tang D; Department of Clinical Medical Research Center, Guangdong Provincial Engineering Research Center of Autoimmune Disease Precision Medicine The Second Clinical Medical College, Jinan University (Shenzhen People's Hospital), Shenzhen, Guangdong, 518020, China.
  • Liu F; Department of Clinical Medical Research Center, Guangdong Provincial Engineering Research Center of Autoimmune Disease Precision Medicine The Second Clinical Medical College, Jinan University (Shenzhen People's Hospital), Shenzhen, Guangdong, 518020, China.
  • Dai Y; The First Affiliated Hospital, Jinan University, 613 W. Huangpu Avenue, Guangzhou, Guangdong, 510632, China.
J Transl Med ; 20(1): 445, 2022 10 02.
Article in En | MEDLINE | ID: mdl-36184622
BACKGROUND: According to the Global Cancer Statistics in 2020, the incidence and mortality of colorectal cancer (CRC) rank third and second among all tumors. The disturbance of ubiquitination plays an important role in the initiation and development of CRC, but the ubiquitinome of CRC cells and the survival-relevant ubiquitination are poorly understood. METHODS: The ubiquitinome of CRC patients (n = 6) was characterized using our own data sets of proteomic and ubiquitin-proteomic examinations. Then, the probable survival-relevant ubiquitination was searched based on the analyses of data sets from public databases. RESULTS: For the ubiquitinomic examination, we identified 1690 quantifiable sites and 870 quantifiable proteins. We found that the highly-ubiquitinated proteins (n ≥ 10) were specifically involved in the biological processes such as G-protein coupling, glycoprotein coupling, and antigen presentation. Also, we depicted five motif sequences frequently recognized by ubiquitin. Subsequently, we revealed that the ubiquitination content of 1172 proteins were up-regulated and 1700 proteins were down-regulated in CRC cells versus normal adjacent cells. We demonstrated that the differentially ubiquitinated proteins were relevant to the pathways including metabolism, immune regulation, and telomere maintenance. Then, integrated with the proteomic datasets from the Clinical Proteomic Tumor Analysis Consortium (CPTAC) (n = 98), we revealed that the increased ubiquitination of FOCAD at Lys583 and Lys587 was potentially associated with patient survival. Finally, we depicted the mutation map of FOCAD and elucidated its potential functions on RNA localization and translation in CRC. CONCLUSIONS: The findings of this study described the ubiquitinome of CRC cells and identified abnormal ubiquitination(s) potentially affecting the patient survival, thereby offering new probable opportunities for clinical treatment.
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Full text: 1 Database: MEDLINE Main subject: Colorectal Neoplasms / Ubiquitinated Proteins Type of study: Diagnostic_studies / Prognostic_studies Limits: Humans Language: En Journal: J Transl Med Year: 2022 Type: Article Affiliation country: China

Full text: 1 Database: MEDLINE Main subject: Colorectal Neoplasms / Ubiquitinated Proteins Type of study: Diagnostic_studies / Prognostic_studies Limits: Humans Language: En Journal: J Transl Med Year: 2022 Type: Article Affiliation country: China