Analytical enantioseparation of N-alkyl drugs by reversed-phase liquid chromatography with carboxymethyl-ß-cyclodextrin as mobile phase additive.
Chirality
; 35(1): 58-66, 2023 01.
Article
in En
| MEDLINE
| ID: mdl-36345792
ABSTRACT
Carboxymethyl-ß-cyclodextrins (CM-ß-CDs) with five kinds of degrees of substitution were synthesized and characterized. Analytical enantioseparation of six basic drugs containing N-alkyl groups, including pheniramine, chlorpheniramine, labetalol, propranolol, venlafaxine, and trans-paroxol, was achieved by reversed-phase high-performance liquid chromatography (RP-HPLC) using the synthesized CM-ß-CD as chiral mobile phase additives. Key influence factors were optimized, including organic modifier, pH value, CM-ß-CD with different degrees of substitution, and concentration of CM-ß-CD. The mobile phase was composed of methanol and 10 mmol L-1 of phosphate buffer pH 4.0 containing 10 mmol L-1 of CM-ß-CD. Peak resolution for six racemic drugs was gradually increased with an increasing degree of substitution of the synthesized CM-ß-CD. The stoichiometric ratio and binding constants for the inclusion complex formed by CM-ß-CD and enantiomer were determined, which showed that the stoichiometric ratio for each inclusion complex was 11.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Beta-Cyclodextrins
/
Chromatography, Reverse-Phase
Language:
En
Journal:
Chirality
Journal subject:
BIOLOGIA MOLECULAR
/
QUIMICA
Year:
2023
Type:
Article
Affiliation country:
China