The Detection and Imaging of pH Values in Living Cells with Hemicyanine Based Colorimetric Mitochondria-Targeted Fluorescent Probe.
J Fluoresc
; 33(1): 393-399, 2023 Jan.
Article
in En
| MEDLINE
| ID: mdl-36434442
ABSTRACT
pH plays a crucial role in cells, especially mitochondria, an important organelle. Developing probes with well-performance for pH detection is still in great demand. Therefore, we first synthesized an indole-based probe (MC-ID-OL) to detect mitochondrial pH changes. The emission wavelength of MC-ID-OL is 649 nm, which does not reach the near-infrared region (650-900 nm). To further enlarge the emission wavelength, probe MC-BI-OL was developed by replacing indolenine with benzoindole. As expected, the emission wavelength changed from 649 to 656 nm. MC-BI-OL probes could also detect pH changes and mitochondria's highly reversible proportional fluorescence localization. In addition, the fluorescence imaging of the MC-BI-OL in HeLa cells demonstrated that this probe could sense changes in the pH of mitochondria in cells.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Colorimetry
/
Fluorescent Dyes
Type of study:
Diagnostic_studies
Limits:
Humans
Language:
En
Journal:
J Fluoresc
Journal subject:
BIOFISICA
Year:
2023
Type:
Article