Imaging of lactate metabolism in retinal Müller cells with a FRET nanosensor.
Exp Eye Res
; 226: 109352, 2023 01.
Article
in En
| MEDLINE
| ID: mdl-36528083
ABSTRACT
Müller cells, the glial cells of the retina, provide metabolic support for photoreceptors and inner retinal neurons, and have been proposed as source of the significant lactate production of this tissue. To better understand the role of lactate in retinal metabolism, we expressed a lactate and a glucose nanosensor in organotypic mouse retinal explants cultured for 14 days, and used FRET imaging in acute vibratome sections of the explants to study metabolite flux in real time. Pharmacological manipulation with specific monocarboxylate transporter (MCT) inhibitors and immunohistochemistry revealed the functional expression of MCT1, MCT2 and MCT4 in Müller cells of retinal explants. The introduction of FRET nanosensors to measure key metabolites at the cellular level may contribute to a better understanding of heretofore poorly understood issues in retinal metabolism.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Fluorescence Resonance Energy Transfer
/
Ependymoglial Cells
Limits:
Animals
Language:
En
Journal:
Exp Eye Res
Year:
2023
Type:
Article
Affiliation country:
Chile