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An ultrasensitive bacterial imprinted electrochemical sensor for the determination of Lactobacillus rhamnosus GG.
Wang, Yue; Zhou, Wen-Yuan; Yang, Zhen-Quan; Jiang, Tie-Min; Song, Jia-Le; Du, Yi-Tian; Gao, Ya-Jun.
Affiliation
  • Wang Y; School of Food Science and Technology, Yangzhou University, Yangzhou, Jiangsu 225127, China.
  • Zhou WY; School of Food Science and Technology, Yangzhou University, Yangzhou, Jiangsu 225127, China.
  • Yang ZQ; School of Food Science and Technology, Yangzhou University, Yangzhou, Jiangsu 225127, China.
  • Jiang TM; South Asia Branch of National Engineering Research Center of Dairy Health for Maternal and Child Health, Guilin University of Technology, Guilin 541004, China. Electronic address: jtm@glut.edu.cn.
  • Song JL; Department of Nutrition and Food Hygiene, Guilin Medical University, Guilin, Guangxi 541004, China; Guangxi Key Laboratory of Enviromental Exposomics and Entire Lifecycle Health, Guilin Medical University, Guilin, Guangxi 541004, China. Electronic address: 12320619@qq.com.
  • Du YT; School of Food Science and Technology, Yangzhou University, Yangzhou, Jiangsu 225127, China.
  • Gao YJ; School of Food Science and Technology, Yangzhou University, Yangzhou, Jiangsu 225127, China. Electronic address: gaoyajun@yzu.edu.cn.
Food Chem ; 410: 135380, 2023 Jun 01.
Article in En | MEDLINE | ID: mdl-36608552
ABSTRACT
An ultrasensitive label-free electrochemical sensor based on a homemade imprinted polypyrrole (PPy) polymer film was prepared to achieve quantitative determination of Lactobacillus rhamnosus GG (LGG). The LGG-imprinted polymer (LIP) film was deposited on a portable screen-printed electrode (SPE) via electropolymerization, which constituted an independent integrated system. The main preparation parameters of the LIP sensor were investigated to obtain optimal performance. Under optimized conditions, the peak current response of the LIP sensor showed a linear relationship with the logarithmic value of LGG concentration in the range from 101 to 109 CFU mL-1 and a detection limit of 5 CFU mL-1. The proposed LIP sensor has achieved efficient, ultrasensitive, highly selective, and cost-effective detection of LGG and can be further developed for practical applications in the quality inspection and development of probiotic products.
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Full text: 1 Database: MEDLINE Main subject: Lacticaseibacillus rhamnosus / Molecular Imprinting Language: En Journal: Food Chem Year: 2023 Type: Article Affiliation country: China
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Full text: 1 Database: MEDLINE Main subject: Lacticaseibacillus rhamnosus / Molecular Imprinting Language: En Journal: Food Chem Year: 2023 Type: Article Affiliation country: China