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Efficient plasmid transfer via natural competence in a microbial co-culture.
Cheng, Yu-Yu; Zhou, Zhichao; Papadopoulos, James M; Zuke, Jason D; Falbel, Tanya G; Anantharaman, Karthik; Burton, Briana M; Venturelli, Ophelia S.
Affiliation
  • Cheng YY; Department of Biochemistry, University of Wisconsin, Madison, WI, USA.
  • Zhou Z; Department of Bacteriology, University of Wisconsin, Madison, WI, USA.
  • Papadopoulos JM; Department of Chemical & Biological Engineering, University of Wisconsin, Madison, WI, USA.
  • Zuke JD; Department of Bacteriology, University of Wisconsin, Madison, WI, USA.
  • Falbel TG; Department of Bacteriology, University of Wisconsin, Madison, WI, USA.
  • Anantharaman K; Department of Bacteriology, University of Wisconsin, Madison, WI, USA.
  • Burton BM; Department of Bacteriology, University of Wisconsin, Madison, WI, USA.
  • Venturelli OS; Department of Biochemistry, University of Wisconsin, Madison, WI, USA.
Mol Syst Biol ; 19(3): e11406, 2023 03 09.
Article in En | MEDLINE | ID: mdl-36714980
ABSTRACT
The molecular and ecological factors shaping horizontal gene transfer (HGT) via natural transformation in microbial communities are largely unknown, which is critical for understanding the emergence of antibiotic-resistant pathogens. We investigate key factors shaping HGT in a microbial co-culture by quantifying extracellular DNA release, species growth, and HGT efficiency over time. In the co-culture, plasmid release and HGT efficiency are significantly enhanced than in the respective monocultures. The donor is a key determinant of HGT efficiency as plasmids induce the SOS response, enter a multimerized state, and are released in high concentrations, enabling efficient HGT. However, HGT is reduced in response to high donor lysis rates. HGT is independent of the donor viability state as both live and dead cells transfer the plasmid with high efficiency. In sum, plasmid HGT via natural transformation depends on the interplay of plasmid properties, donor stress responses and lysis rates, and interspecies interactions.
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Full text: 1 Database: MEDLINE Main subject: DNA / Anti-Bacterial Agents Language: En Journal: Mol Syst Biol Journal subject: BIOLOGIA MOLECULAR / BIOTECNOLOGIA Year: 2023 Type: Article Affiliation country: United States

Full text: 1 Database: MEDLINE Main subject: DNA / Anti-Bacterial Agents Language: En Journal: Mol Syst Biol Journal subject: BIOLOGIA MOLECULAR / BIOTECNOLOGIA Year: 2023 Type: Article Affiliation country: United States