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Determination of 24,25-dihydroxyvitamin D3 in Vitamin D External Quality Assessment Scheme samples using a reference measurement procedure.
Wise, Stephen A; Hahm, Grace; Burdette, Carolyn Q; Tai, Susan S-C; Camara, Johanna E; Sempos, Christopher T; Williams, Emma L.
Affiliation
  • Wise SA; Office of Dietary Supplements, National Institutes of Health, Bethesda, MD 20892, USA. Electronic address: stephen.wise@nih.gov.
  • Hahm G; National Institute of Standards and Technology (NIST), Gaithersburg, MD 20899, USA.
  • Burdette CQ; National Institute of Standards and Technology (NIST), Gaithersburg, MD 20899, USA.
  • Tai SS; National Institute of Standards and Technology (NIST), Gaithersburg, MD 20899, USA.
  • Camara JE; National Institute of Standards and Technology (NIST), Gaithersburg, MD 20899, USA.
  • Sempos CT; Vitamin D Standardization Program LLC, Havre de Grace, MD 21078, USA.
  • Williams EL; Imperial Healthcare NHS Trust, London W6 8RF, UK.
J Steroid Biochem Mol Biol ; 231: 106318, 2023 07.
Article in En | MEDLINE | ID: mdl-37169270
ABSTRACT
Ninety archived human serum samples from the Vitamin D External Quality Assessment Scheme (DEQAS) were analyzed using a reference measurement procedure (RMP) based on isotope dilution liquid chromatography - tandem mass spectrometry (ID LC-MS/MS) for the determination of 24,25-dihydroxyvitamin D3 [24,25(OH)2D3]. These 24,25(OH)2D3 results, in conjunction with concentration values assigned using RMPs for 25-hydroxyvitamin D2 [25(OH)D2] and 25-hydroxyvitamin D3 [25(OH)D3], provide a valuable resource for assessing the accuracy of measurements for 24,25(OH)2D3 and for investigating the relationship between 24,25(OH)2D3 and 25(OH)D3. Results for 24,25(OH)2D3 using the RMP were compared to DEQAS consensus values demonstrating that the consensus values were not sufficient to assess the accuracy of measurements among different laboratories and methods. A multivariable regression analysis approach using historical DEQAS consensus values for various total 25(OH)D assays was used to assess the contribution of 24,25(OH)2D3 concentration on the assay response. The response of several ligand binding assays for total 25(OH)D was shown to be impacted by the presence of 24,25(OH)2D3.
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Full text: 1 Database: MEDLINE Main subject: Vitamin D / Tandem Mass Spectrometry Limits: Humans Language: En Journal: J Steroid Biochem Mol Biol Journal subject: BIOLOGIA MOLECULAR / BIOQUIMICA Year: 2023 Type: Article
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Full text: 1 Database: MEDLINE Main subject: Vitamin D / Tandem Mass Spectrometry Limits: Humans Language: En Journal: J Steroid Biochem Mol Biol Journal subject: BIOLOGIA MOLECULAR / BIOQUIMICA Year: 2023 Type: Article