Maturing differentiated human pluripotent stem cells in vitro: methods and challenges.
Development
; 150(11)2023 06 01.
Article
in En
| MEDLINE
| ID: mdl-37260361
ABSTRACT
Human pluripotent stem cells (hPSCs), derived from individuals or genetically modified with disease-related mutations and variants, have revolutionised studies of human disease. Researchers are beginning to exploit the extraordinary potential of stem cell technology to screen for new drugs to treat intractable diseases, ideally without side-effects. However, a major problem is that the differentiated cell types on which these models are based are immature; they resemble fetal and not adult cells. Here, we discuss the nature and hurdles of hPSC maturation, using cardiomyocytes as an example. We review methods used to induce cardiomyocyte maturation in culture and consider remaining challenges for their integration into research on human disease and drug development pipelines.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Pluripotent Stem Cells
/
Induced Pluripotent Stem Cells
Limits:
Humans
Language:
En
Journal:
Development
Journal subject:
BIOLOGIA
/
EMBRIOLOGIA
Year:
2023
Type:
Article
Affiliation country:
Italy