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Conditional replication and secretion of hepatitis B virus genome uncover the truncated 3' terminus of encapsidated viral pregenomic RNA.
Shen, Sheng; Liu, Wendong; Zeng, Ge; Liang, Hongyan; Yu, Xiaoyang; Zhang, Hu; Sun, Jian; Guo, Haitao.
Affiliation
  • Shen S; Department of Infectious Diseases, State Key Laboratory of Organ Failure Research, Key Laboratory of Infectious Diseases Research in South China, Ministry of Education, Guangdong Provincial Key Laboratory of Viral Hepatitis Research, Nanfang Hospital, Southern Medical University , Guangzhou, China.
  • Liu W; Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine , Pittsburgh, Pennsylvania, USA.
  • Zeng G; Cancer Virology Program, UPMC Hillman Cancer Center, University of Pittsburgh School of Medicine , Pittsburgh, Pennsylvania, USA.
  • Liang H; Department of Infectious Diseases, State Key Laboratory of Organ Failure Research, Key Laboratory of Infectious Diseases Research in South China, Ministry of Education, Guangdong Provincial Key Laboratory of Viral Hepatitis Research, Nanfang Hospital, Southern Medical University , Guangzhou, China.
  • Yu X; Department of Infectious Diseases, State Key Laboratory of Organ Failure Research, Key Laboratory of Infectious Diseases Research in South China, Ministry of Education, Guangdong Provincial Key Laboratory of Viral Hepatitis Research, Nanfang Hospital, Southern Medical University , Guangzhou, China.
  • Zhang H; Department of Infectious Diseases, State Key Laboratory of Organ Failure Research, Key Laboratory of Infectious Diseases Research in South China, Ministry of Education, Guangdong Provincial Key Laboratory of Viral Hepatitis Research, Nanfang Hospital, Southern Medical University , Guangzhou, China.
  • Sun J; Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine , Pittsburgh, Pennsylvania, USA.
  • Guo H; Cancer Virology Program, UPMC Hillman Cancer Center, University of Pittsburgh School of Medicine , Pittsburgh, Pennsylvania, USA.
J Virol ; 97(10): e0076023, 2023 10 31.
Article in En | MEDLINE | ID: mdl-37754759
ABSTRACT
IMPORTANCE The biogenesis and clinical application of serum HBV pgRNA have been a research hotspot in recent years. This study further characterized the heterogeneity of the 3' terminus of capsid RNA by utilizing a variety of experimental systems conditionally supporting HBV genome replication and secretion, and reveal that the 3' truncation of capsid pgRNA is catalyzed by cellular ribonuclease(s) and viral RNaseH at positions after and before 3' DR1, respectively, indicating the 3' DR1 as a boundary between the encapsidated portion of pgRNA for reverse transcription and the 3' unprotected terminus, which is independent of pgRNA length and the 3' terminal sequence. Thus, our study provides new insights into the mechanism of pgRNA encapsidation and reverse transcription, as well as the optimization of serum HBV RNA diagnostics.
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Full text: 1 Database: MEDLINE Main subject: Virus Replication / RNA, Viral / Hepatitis B virus / Capsid / Genome, Viral Language: En Journal: J Virol Year: 2023 Type: Article Affiliation country: China
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Full text: 1 Database: MEDLINE Main subject: Virus Replication / RNA, Viral / Hepatitis B virus / Capsid / Genome, Viral Language: En Journal: J Virol Year: 2023 Type: Article Affiliation country: China