Comparative cryopreservation of indian wild orange (Citrus indica Tanaka) embryonic axes.
Cryo Letters
; 44(3): 142-150, 2023.
Article
in En
| MEDLINE
| ID: mdl-37883167
ABSTRACT
BACKGROUND:
Indian Wild Orange (Citrus indica Tanaka) is an endangered and endemic species from northeast India for which effective ex situ conservation strategies, including embryo cryopreservation, are urgently needed. MATERIALS ANDMETHODS:
Desiccation tolerance and cryopreservation ability for embryonic axes of Citrus indica was determined using three techniques (air desiccation-freezing, PVS2 vitrification-freezing and encapsulation-dehydration-freezing). Success was assessed as survival and recovery in vitro.RESULTS:
Successful cryopreservation of embryonic axes was achieved using all three methods, with the highest survival achieved when using air desiccation-freezing (90%) followed by encapsulation-dehydration (85%) and PVS2 vitrification cryopreservation (80%). Regeneration levels were lower than survival levels for all three proceedures. Post-cryo regeneration success was encapsulation-dehydration (64%) > air desiccation-freezing (55%) > PVS2 vitrification (52%).CONCLUSION:
Although there was relatively high post-cryopreservation recovery growth obtained using all the three techniques, the air desiccation-freezing technique is preferred, as it is a simple, practical and reproducible technique for the long-term cryobanking of this important wild species. Doi 10.54680/fr23310110512.
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Database:
MEDLINE
Main subject:
Cryopreservation
/
Citrus
Language:
En
Journal:
Cryo Letters
Journal subject:
BIOLOGIA
/
QUIMICA
Year:
2023
Type:
Article
Affiliation country:
India