A dissected non-ribosomal peptide synthetase maintains activity.
Biochim Biophys Acta Proteins Proteom
; 1872(1): 140972, 2024 01 01.
Article
in En
| MEDLINE
| ID: mdl-37951518
ABSTRACT
Non-ribosomal peptide synthetases (NRPSs) generate chemically complex compounds and their modular architecture suggests that changing their domain organization can predictably alter their products. Ebony, a small three-domain NRPS, catalyzes the formation of ß-alanine containing amides from biogenic amines. To examine the necessity of interdomain interactions, we modeled and docked domains of Ebony to reveal potential interfaces between them. Testing the same domain combinations in vitro showed that 8 % of activity was preserved after Ebony was dissected into a di-domain and a detached C-terminal domain, suggesting that sufficient interaction was maintained after dissection. Our work creates a model to identify domain interfaces necessary for catalysis, an important step toward utilizing Ebony as a combinatorial engineering platform for novel amides.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Peptide Synthases
/
Amides
Language:
En
Journal:
Biochim Biophys Acta Proteins Proteom
Year:
2024
Type:
Article
Affiliation country:
United States