Existence and importance of Na+K+-ATPase in the plasma membrane of boar spermatozoa.
Can J Physiol Pharmacol
; 102(4): 254-269, 2024 Apr 01.
Article
in En
| MEDLINE
| ID: mdl-38029410
ABSTRACT
Sodium-potassium-ATPase (Na+K+-ATPase), a target to treat congestive heart failure, is the only known receptor for cardiac glycosides implicated in intracellular signaling and additionally functions enzymatically in ion transport. Spermatozoa need transmembrane ion transport and signaling to fertilize, and Na+K+-ATPase is identified here for the first time in boar spermatozoa. Head plasma membrane (HPM) isolated from boar spermatozoa was confirmed pure by marker enzymes acid and alkaline phosphatase (218 ± 23% and 245 ± 38% enrichment, respectively, versus whole spermatozoa). Western immunoblotting detected α and ß subunits (isoforms α1, α3, ß1, ß2, and ß3) in different concentrations in whole spermatozoa and HPM. Immunofluorescence of intact sperm only detected α3 on the post-equatorial exterior membrane; methanol-permeabilized sperm also had α3 post-equatorially and other isoforms on the acrosomal ridge and cap. Mass spectrometry confirmed the presence of all isoforms in HPM. Incubating boar sperm in capacitating media to induce the physiological changes preceding fertilization significantly increased the percentage of capacitated sperm compared to 0 h control (33.0 ± 2.6% vs. 19.2 ± 2.6% capacitated sperm, respectively; p = 0.014) and altered the ß2 immunofluorescence pattern. These results demonstrate the presence of Na+K+-ATPase in boar sperm HPM and that it changes during capacitation.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Semen
/
Sperm Capacitation
Limits:
Animals
Language:
En
Journal:
Can J Physiol Pharmacol
Year:
2024
Type:
Article
Affiliation country:
Canada