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Multilocus Distance-Regulated Sensor Array for Recognition of Polyphenols via Machine Learning and Indicator Displacement Assay.
Ni, Weiwei; Yu, Yang; Gao, Xu; Han, Yang; Zhang, Wenhui; Zhang, Zerui; Xiao, Wenqi; Hu, Qin; Zhang, Yanliang; Huang, Hui; Li, Fei; Chen, Mingqi; Han, Jinsong.
Affiliation
  • Ni W; State Key Laboratory of Natural Medicines, College of Engineering, China Pharmaceutical University, Nanjing 211109, China.
  • Yu Y; School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211109, China.
  • Gao X; State Key Laboratory of Natural Medicines, College of Engineering, China Pharmaceutical University, Nanjing 211109, China.
  • Han Y; School of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University, Nanjing 211109, China.
  • Zhang W; State Key Laboratory of Natural Medicines, College of Engineering, China Pharmaceutical University, Nanjing 211109, China.
  • Zhang Z; State Key Laboratory of Natural Medicines, College of Engineering, China Pharmaceutical University, Nanjing 211109, China.
  • Xiao W; State Key Laboratory of Natural Medicines, College of Engineering, China Pharmaceutical University, Nanjing 211109, China.
  • Hu Q; Department of Laboratory Medicine, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China.
  • Zhang Y; Nanjing Research Center for Infectious Diseases of Integrated Traditional Chinese and Western Medicine, Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Nanjing 211109, China.
  • Huang H; State Key Laboratory of Natural Medicines, College of Engineering, China Pharmaceutical University, Nanjing 211109, China.
  • Li F; State Key Laboratory of Natural Medicines, College of Engineering, China Pharmaceutical University, Nanjing 211109, China.
  • Chen M; State Key Laboratory of Natural Medicines, College of Engineering, China Pharmaceutical University, Nanjing 211109, China.
  • Han J; State Key Laboratory of Natural Medicines, College of Engineering, China Pharmaceutical University, Nanjing 211109, China.
Anal Chem ; 96(1): 301-308, 2024 01 09.
Article in En | MEDLINE | ID: mdl-38102984
ABSTRACT
Developing new strategies to construct sensor arrays that can effectively distinguish multiple natural components with similar structures in mixtures is an exceptionally challenging task. Here, we propose a new multilocus distance-modulated indicator displacement assay (IDA) strategy for constructing a sensor array, incorporating machine learning optimization to identify polyphenols. An 8-element array, comprising two fluorophores and their six dynamic covalent complexes (C1-C6) formed by pairing two fluorophores with three distinct distance-regulated quenchers, has been constructed. Polyphenols with diverse spatial arrangements and combinatorial forms compete with the fluorophores by forming pseudocycles with quenchers within the complexes, leading to varying degrees of fluorescence recovery. The array accurately and effectively distinguished four tea polyphenols and 16 tea varieties, thereby demonstrating the broad applicability of the multilocus distance-modulated IDA array in detecting polyhydroxy foods and natural medicines.
Subject(s)

Full text: 1 Database: MEDLINE Main subject: Tea / Polyphenols Language: En Journal: Anal Chem Year: 2024 Type: Article Affiliation country: China

Full text: 1 Database: MEDLINE Main subject: Tea / Polyphenols Language: En Journal: Anal Chem Year: 2024 Type: Article Affiliation country: China