Fusion-negative rhabdomyosarcoma 3D organoids to predict effective drug combinations: A proof-of-concept on cell death inducers.
Cell Rep Med
; 4(12): 101339, 2023 12 19.
Article
in En
| MEDLINE
| ID: mdl-38118405
ABSTRACT
Rhabdomyosarcoma (RMS) is the main form of pediatric soft-tissue sarcoma. Its cure rate has not notably improved in the last 20 years following relapse, and the lack of reliable preclinical models has hampered the design of new therapies. This is particularly true for highly heterogeneous fusion-negative RMS (FNRMS). Although methods have been proposed to establish FNRMS organoids, their efficiency remains limited to date, both in terms of derivation rate and ability to accurately mimic the original tumor. Here, we present the development of a next-generation 3D organoid model derived from relapsed adult and pediatric FNRMS. This model preserves the molecular features of the patients' tumors and is expandable for several months in 3D, reinforcing its interest to drug combination screening with longitudinal efficacy monitoring. As a proof-of-concept, we demonstrate its preclinical relevance by reevaluating the therapeutic opportunities of targeting apoptosis in FNRMS from a streamlined approach based on transcriptomic data exploitation.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Rhabdomyosarcoma
/
Antineoplastic Agents
Limits:
Adult
/
Child
/
Humans
Language:
En
Journal:
Cell Rep Med
Year:
2023
Type:
Article
Affiliation country:
France