Cell via Cell Viability Assay Changes Cellular Metabolic Characteristics by Intervening with Glycolysis and Pentose Phosphate Pathway.
Chem Res Toxicol
; 37(2): 208-211, 2024 02 19.
Article
in En
| MEDLINE
| ID: mdl-38191130
ABSTRACT
The Cell Counting Kit-8 (CCK-8) cell viability assay, also known as WST-8, is widely recognized for its nontoxic nature, making it suitable for further studies on treated cells. This practice is commonly observed in the field of tissue engineering. While live/dead imaging may not readily reveal macroscopic differences, our investigation has uncovered significant intracellular metabolic changes. Notably, we observed substantial down-regulation of metabolites within the glycolysis and pentose phosphate pathways. These metabolic alterations predominantly affect energy metabolism and may potentially impact the cellular redox environment. In light of these findings, we strongly recommend that researchers exercise caution when using cells treated with CCK-8 in subsequent experiments.
Full text:
1
Database:
MEDLINE
Main subject:
Pentose Phosphate Pathway
/
Glycolysis
Language:
En
Journal:
Chem Res Toxicol
/
Chem. res. toxicol
/
Chemical research in toxicology
Journal subject:
TOXICOLOGIA
Year:
2024
Type:
Article
Affiliation country:
Latvia