Controlled exchange of protein and nucleic acid signals from and between synthetic minimal cells.
Cell Syst
; 15(1): 49-62.e4, 2024 01 17.
Article
in En
| MEDLINE
| ID: mdl-38237551
ABSTRACT
Synthetic minimal cells are a class of bioreactors that have some, but not all, functions of live cells. Here, we report a critical step toward the development of a bottom-up minimal cell cellular export of functional protein and RNA products. We used cell-penetrating peptide tags to translocate payloads across a synthetic cell vesicle membrane. We demonstrated efficient transport of active enzymes and transport of nucleic acid payloads by RNA-binding proteins. We investigated influence of a concentration gradient alongside other factors on the efficiency of the translocation, and we show a method to increase product accumulation in one location. We demonstrate the use of this technology to engineer molecular communication between different populations of synthetic cells, to exchange protein and nucleic acid signals. The synthetic minimal cell production and export of proteins or nucleic acids allows experimental designs that approach the complexity and relevancy of natural biological systems. A record of this paper's transparent peer review process is included in the supplemental information.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Nucleic Acids
/
Cell-Penetrating Peptides
/
Artificial Cells
Language:
En
Journal:
Cell Syst
Year:
2024
Type:
Article
Affiliation country:
United States