Protocol to visualize trans-interaction of clustered protocadherin using cIPAD, a fluorescent indicator, in cultured human cells and mouse neurons.
STAR Protoc
; 5(1): 102844, 2024 Mar 15.
Article
in En
| MEDLINE
| ID: mdl-38277267
ABSTRACT
cIPAD is a fluorescent indicator that allows the visualization of trans-interactions of clustered protocadherin (Pcdh), a cell adhesion molecule that mediates neuronal self-recognition. We describe steps for using HEK293T cells to visualize Pcdh trans-interactions across cells as a preliminary experiment before using dissociated mouse neurons. We then detail procedures for visualizing Pcdh trans-interactions between processes originating from the same neurons, which are considered as Pcdh-mediated neuronal self-recognition. For complete details on the use and execution of this protocol, please refer to Kanadome et al.1.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Cadherins
/
Protocadherins
Limits:
Animals
/
Humans
Language:
En
Journal:
STAR Protoc
Year:
2024
Type:
Article