Stable-Isotope N-Me Aziridination Enables Accurate Quantitative CâC Isomeric Lipidomics.
Anal Chem
; 96(6): 2524-2533, 2024 02 13.
Article
in En
| MEDLINE
| ID: mdl-38308578
ABSTRACT
Accurate lipid quantification is essential to revealing their roles in physiological and pathological processes. However, difficulties in the structural resolution of lipid isomers hinder their further accurate quantification. To address this challenge, we developed a novel stable-isotope N-Me aziridination strategy that enables simultaneous qualification and quantification of unsaturated lipid isomers. The one-step introduction of the 1-methylaziridine structure not only serves as an activating group for the CâC bond to facilitate positional identification but also as an isotopic inserter to achieve accurate relative quantification. The high performance of this reaction for the identification of unsaturated lipids was verified by large-scale resolution of the CâC positions of 468 lipids in serum. More importantly, by using this bifunctional duplex labeling method, various unsaturated lipids such as fatty acids, phospholipids, glycerides, and cholesterol ester were accurately and individually quantified at the CâC bond isomeric level during the mouse brain ischemia. This study provides a new approach to quantitative structural lipidomics.
Full text:
1
Database:
MEDLINE
Main subject:
Fatty Acids
/
Lipidomics
Limits:
Animals
Language:
En
Journal:
Anal Chem
Year:
2024
Type:
Article