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Fibrosis and expression of extracellular matrix proteins in human interventricular septum in aortic valve stenosis and regurgitation.
Sedmera, David; Kvasilova, Alena; Eckhardt, Adam; Kacer, Petr; Penicka, Martin; Kocka, Matej; Schindler, Dana; Kaban, Ron; Kockova, Radka.
Affiliation
  • Sedmera D; Institute of Anatomy, First Faculty of Medicine, Charles University, U Nemocnice 3, 128 00, Prague, Czech Republic. david.sedmera@lf1.cuni.cz.
  • Kvasilova A; Institute of Physiology, The Czech Academy of Sciences, Videnska 1024, 142 00, Prague, Czech Republic. david.sedmera@lf1.cuni.cz.
  • Eckhardt A; Institute of Anatomy, First Faculty of Medicine, Charles University, U Nemocnice 3, 128 00, Prague, Czech Republic.
  • Kacer P; Institute of Physiology, The Czech Academy of Sciences, Videnska 1024, 142 00, Prague, Czech Republic.
  • Penicka M; Department of Cardiac Surgery, Third Faculty of Medicine, Charles University, Prague, Czech Republic.
  • Kocka M; University Hospital Kralovske Vinohrady, Prague, Czech Republic.
  • Schindler D; Cardiovascular Center Aalst, OLV Clinic, 9300, Aalst, Belgium.
  • Kaban R; Institute of Anatomy, First Faculty of Medicine, Charles University, U Nemocnice 3, 128 00, Prague, Czech Republic.
  • Kockova R; Institute of Anatomy, First Faculty of Medicine, Charles University, U Nemocnice 3, 128 00, Prague, Czech Republic.
Histochem Cell Biol ; 161(5): 367-379, 2024 May.
Article in En | MEDLINE | ID: mdl-38347221
ABSTRACT
Valvular heart disease leads to ventricular pressure and/or volume overload. Pressure overload leads to fibrosis, which might regress with its resolution, but the limits and details of this reverse remodeling are not known. To gain more insight into the extent and nature of cardiac fibrosis in valve disease, we analyzed needle biopsies taken from the interventricular septum of patients undergoing surgery for valve replacement focusing on the expression and distribution of major extracellular matrix protein involved in this process. Proteomic analysis performed using mass spectrometry revealed an excellent correlation between the expression of collagen type I and III, but there was little correlation with the immunohistochemical staining performed on sister sections, which included antibodies against collagen I, III, fibronectin, sarcomeric actin, and histochemistry for wheat germ agglutinin. Surprisingly, the immunofluorescence intensity did not correlate significantly with the gold standard for fibrosis quantification, which was performed using Picrosirius Red (PSR) staining, unless multiplexed on the same tissue section. There was also little correlation between the immunohistochemical markers and pressure gradient severity. It appears that at least in humans, the immunohistochemical pattern of fibrosis is not clearly correlated with standard Picrosirius Red staining on sister sections or quantitative proteomic data, possibly due to tissue heterogeneity at microscale, comorbidities, or other patient-specific factors. For precise correlation of different types of staining, multiplexing on the same section is the best approach.
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Full text: 1 Database: MEDLINE Main subject: Aortic Valve Stenosis / Fibrosis / Extracellular Matrix Proteins Limits: Aged / Female / Humans / Male / Middle aged Language: En Journal: Histochem Cell Biol Journal subject: CITOLOGIA / HISTOCITOQUIMICA Year: 2024 Type: Article Affiliation country: Czech Republic

Full text: 1 Database: MEDLINE Main subject: Aortic Valve Stenosis / Fibrosis / Extracellular Matrix Proteins Limits: Aged / Female / Humans / Male / Middle aged Language: En Journal: Histochem Cell Biol Journal subject: CITOLOGIA / HISTOCITOQUIMICA Year: 2024 Type: Article Affiliation country: Czech Republic