Localized Plasmonic Heating for Single-Molecule DNA Rupture Measurements in Optical Tweezers.
Nano Lett
; 24(10): 3097-3103, 2024 Mar 13.
Article
in En
| MEDLINE
| ID: mdl-38417053
ABSTRACT
To date, studies on the thermodynamic and kinetic processes that underlie biological function and nanomachine actuation in biological- and biology-inspired molecular constructs have primarily focused on photothermal heating of ensemble systems, highlighting the need for probes that are localized within the molecular construct and capable of resolving single-molecule response. Here we present an experimental demonstration of wavelength-selective, localized heating at the single-molecule level using the surface plasmon resonance of a 15 nm gold nanoparticle (AuNP). Our approach is compatible with force-spectroscopy measurements and can be applied to studies of the single-molecule thermodynamic properties of DNA origami nanomachines as well as biomolecular complexes. We further demonstrate wavelength selectivity and establish the temperature dependence of the reaction coordinate for base-pair disruption in the shear-rupture geometry, demonstrating the utility and flexibility of this approach for both fundamental studies of local (nanometer-scale) temperature gradients and rapid and multiplexed nanomachine actuation.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Metal Nanoparticles
/
Gold
Language:
En
Journal:
Nano Lett
Year:
2024
Type:
Article
Affiliation country:
United States