Heterogeneity of SOS response expression in clinical isolates of Escherichia coli influences adaptation to antimicrobial stress.
Drug Resist Updat
; 75: 101087, 2024 Jul.
Article
in En
| MEDLINE
| ID: mdl-38678745
ABSTRACT
In recent years, new evidence has shown that the SOS response plays an important role in the response to antimicrobials, with involvement in the generation of clinical resistance. Here we evaluate the impact of heterogeneous expression of the SOS response in clinical isolates of Escherichia coli on response to the fluoroquinolone, ciprofloxacin. In silico analysis of whole genome sequencing data showed remarkable sequence conservation of the SOS response regulators, RecA and LexA. Despite the genetic homogeneity, our results revealed a marked differential heterogeneity in SOS response activation, both at population and single-cell level, among clinical isolates of E. coli in the presence of subinhibitory concentrations of ciprofloxacin. Four main stages of SOS response activation were identified and correlated with cell filamentation. Interestingly, there was a correlation between clinical isolates with higher expression of the SOS response and further progression to resistance. This heterogeneity in response to DNA damage repair (mediated by the SOS response) and induced by antimicrobial agents could be a new factor with implications for bacterial evolution and survival contributing to the generation of antimicrobial resistance.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Rec A Recombinases
/
SOS Response, Genetics
/
Ciprofloxacin
/
Microbial Sensitivity Tests
/
Escherichia coli Proteins
/
Escherichia coli
/
Anti-Bacterial Agents
Limits:
Humans
Language:
En
Journal:
Drug Resist Updat
Journal subject:
ANTINEOPLASICOS
Year:
2024
Type:
Article