Evaluating an extraction-free sample preparation method for multiplex detection of SARS-Cov-2, influenza A/B, and RSV with implementation on a microfluidic chip.
Diagn Microbiol Infect Dis
; 109(3): 116325, 2024 Jul.
Article
in En
| MEDLINE
| ID: mdl-38688146
ABSTRACT
Following the relaxation of COVID-19 restrictions, other respiratory viruses such as influenza and respiratory syncytial virus (RSV), whose transmission were decreased due to COVID-19 precautions, are rising again. Because of similar clinical features and reported co-infections, multiplex detection of SARS-CoV-2, influenza A/B, and RSV is required to use specific treatments. This study assessed an extraction-free sample preparation (heat treatment at 95°C for 3 minutes) for multiplex detection using rRT-PCR. Despite an observed Ct-delay (∆Ct) averageing 1.26 compared to the standard method, an acceptable total sensitivity of 92 % and a negative predictive value (NPV) of 96 % were obtained. Moreover, Implementation on a microfluidic chip demonstrated efficiency, maintaining an excellent correlation (R2=0.983) with the standard method. Combining this extraction-free procedure with rRT-PCR on a microfluidic chip seems promising, because it simplifies the design and reduces the cost and complexity of the integrated assay for multiplex detection of SARS-CoV-2, influenza A/B, and RSV.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Influenza A virus
/
Influenza B virus
/
Respiratory Syncytial Virus Infections
/
Influenza, Human
/
SARS-CoV-2
/
COVID-19
Limits:
Humans
Language:
En
Journal:
Diagn Microbiol Infect Dis
Year:
2024
Type:
Article
Affiliation country:
Iran