Generation of Defective Interfering Particles of Morbilliviruses Using Reverse Genetics.
Methods Mol Biol
; 2808: 57-70, 2024.
Article
in En
| MEDLINE
| ID: mdl-38743362
ABSTRACT
RNA viruses generate defective genomes naturally during virus replication. Defective genomes that interfere with the infection dynamics either through resource competition or by interferon stimulation are known as defective interfering (DI) genomes. DI genomes can be successfully packaged into virus-like-particles referred to as defective interfering particles (DIPs). Such DIPs can sustainably coexist with the full-length virus particles and have been shown to negatively impact virus replication in vitro and in vivo. Here, we describe a method to generate a clonal DI genome population by reverse genetics. This method is applicable to other RNA viruses and will enable assessment of DIPs for their antiviral properties.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Virus Replication
/
Genome, Viral
/
Morbillivirus
/
Defective Viruses
/
Reverse Genetics
Limits:
Animals
/
Humans
Language:
En
Journal:
Methods Mol Biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2024
Type:
Article
Affiliation country:
United States