Endonuclease Q as a robust enhancer for nucleic acid amplification.
Anal Biochem
; 692: 115569, 2024 Sep.
Article
in En
| MEDLINE
| ID: mdl-38750682
ABSTRACT
Isothermal nucleic acid amplification techniques are attracting increasing attention in molecular diagnosis and biotechnology. However, most existing techniques are complicated by the need for intricate primer design and numerous enzymes and primers. Here, we have developed a simple method, termed NAQ, that employs adding both endonuclease Q (EndoQ) and dUTP/dITP to conventional rolling circle amplification reactions to increase DNA amplification. NAQ does not require intricate primer design or DNA sequence-specific enzymes, and existing isothermal amplification techniques could be readily adapted to include both EndoQ and dUTP/dITP.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Nucleic Acid Amplification Techniques
Language:
En
Journal:
Anal Biochem
Year:
2024
Type:
Article