Azithromycin resistance in Escherichia coli and Salmonella from food-producing animals and meat in Europe.
J Antimicrob Chemother
; 79(7): 1657-1667, 2024 07 01.
Article
in En
| MEDLINE
| ID: mdl-38775752
ABSTRACT
OBJECTIVES:
To characterize the genetic basis of azithromycin resistance in Escherichia coli and Salmonella collected within the EU harmonized antimicrobial resistance (AMR) surveillance programme in 2014-18 and the Danish AMR surveillance programme in 2016-19.METHODS:
WGS data of 1007 E. coli [165 azithromycin resistant (MICâ>â16â mg/L)] and 269 Salmonella [29 azithromycin resistant (MICâ>â16â mg/L)] were screened for acquired macrolide resistance genes and mutations in rplDV, 23S rRNA and acrB genes using ResFinder v4.0, AMRFinder Plus and custom scripts. Genotype-phenotype concordance was determined for all isolates. Transferability of mef(C)-mph(G)-carrying plasmids was assessed by conjugation experiments.RESULTS:
mph(A), mph(B), mef(B), erm(B) and mef(C)-mph(G) were detected in E. coli and Salmonella, whereas erm(C), erm(42), ere(A) and mph(E)-msr(E) were detected in E. coli only. The presence of macrolide resistance genes, alone or in combination, was concordant with the azithromycin-resistant phenotype in 69% of isolates. Distinct mph(A) operon structures were observed in azithromycin-susceptible (nâ=â50) and -resistant (nâ=â136) isolates. mef(C)-mph(G) were detected in porcine and bovine E. coli and in porcine Salmonella enterica serovar Derby and Salmonella enterica 1,4, [5],12i-, flanked downstream by ISCR2 or TnAs1 and associated with IncIγ and IncFII plasmids.CONCLUSIONS:
Diverse azithromycin resistance genes were detected in E. coli and Salmonella from food-producing animals and meat in Europe. Azithromycin resistance genes mef(C)-mph(G) and erm(42) appear to be emerging primarily in porcine E. coli isolates. The identification of distinct mph(A) operon structures in susceptible and resistant isolates increases the predictive power of WGS-based methods for in silico detection of azithromycin resistance in Enterobacterales.
Full text:
1
Database:
MEDLINE
Main subject:
Salmonella
/
Microbial Sensitivity Tests
/
Azithromycin
/
Drug Resistance, Bacterial
/
Escherichia coli
/
Meat
/
Anti-Bacterial Agents
Limits:
Animals
Country/Region as subject:
Europa
Language:
En
Journal:
J Antimicrob Chemother
Year:
2024
Type:
Article
Affiliation country:
Denmark