Sphingosine-1-phosphate Decreases Erythrocyte Dysfunction Induced by ß-Amyloid.
Int J Mol Sci
; 25(10)2024 May 10.
Article
in En
| MEDLINE
| ID: mdl-38791223
ABSTRACT
Amyloid beta peptides (Aß) have been identified as the main pathogenic agents in Alzheimer's disease (AD). Soluble Aß oligomers, rather than monomer or insoluble amyloid fibrils, show red blood cell (RBC) membrane-binding capacity and trigger several morphological and functional alterations in RBCs that can result in impaired oxygen transport and delivery. Since bioactive lipids have been recently proposed as potent protective agents against Aß toxicity, we investigated the role of sphingosine-1-phosphate (S1P) in signaling pathways involved in the mechanism underlying ATP release in Ab-treated RBCs. In RBCs following different treatments, the ATP, 2,3 DPG and cAMP levels and caspase 3 activity were determined by spectrophotometric and immunoassay. S1P rescued the inhibition of ATP release from RBCs triggered by Ab, through a mechanism involving caspase-3 and restoring 2,3 DPG and cAMP levels within the cell. These findings reveal the molecular basis of S1P protection against Aß in RBCs and suggest new therapeutic avenues in AD.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Sphingosine
/
Lysophospholipids
/
Adenosine Triphosphate
/
Amyloid beta-Peptides
/
Cyclic AMP
/
Erythrocytes
/
Caspase 3
Limits:
Humans
Language:
En
Journal:
Int J Mol Sci
Year:
2024
Type:
Article
Affiliation country:
Italy