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Systematic engineering for production of anti-aging sunscreen compound in Pseudomonas putida.
Yunus, Ian S; Hudson, Graham A; Chen, Yan; Gin, Jennifer W; Kim, Joonhoon; Baidoo, Edward E K; Petzold, Christopher J; Adams, Paul D; Simmons, Blake A; Mukhopadhyay, Aindrila; Keasling, Jay D; Lee, Taek Soon.
Affiliation
  • Yunus IS; Joint BioEnergy Institute, 5885 Hollis Street, Emeryville, CA, USA; Biological Systems & Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA. Electronic address: iansofianyunus@gmail.com.
  • Hudson GA; Joint BioEnergy Institute, 5885 Hollis Street, Emeryville, CA, USA; Biological Systems & Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA; California Institute of Quantitative Biosciences (QB3), University of California, Berkeley, CA, USA.
  • Chen Y; Joint BioEnergy Institute, 5885 Hollis Street, Emeryville, CA, USA; Biological Systems & Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
  • Gin JW; Joint BioEnergy Institute, 5885 Hollis Street, Emeryville, CA, USA; Biological Systems & Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
  • Kim J; Joint BioEnergy Institute, 5885 Hollis Street, Emeryville, CA, USA; Energy Processes & Materials Division, Pacific Northwest National Laboratory, Richland, WA, USA.
  • Baidoo EEK; Joint BioEnergy Institute, 5885 Hollis Street, Emeryville, CA, USA; Biological Systems & Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
  • Petzold CJ; Joint BioEnergy Institute, 5885 Hollis Street, Emeryville, CA, USA; Biological Systems & Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
  • Adams PD; Joint BioEnergy Institute, 5885 Hollis Street, Emeryville, CA, USA; Molecular Biophysics & Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
  • Simmons BA; Joint BioEnergy Institute, 5885 Hollis Street, Emeryville, CA, USA; Biological Systems & Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
  • Mukhopadhyay A; Joint BioEnergy Institute, 5885 Hollis Street, Emeryville, CA, USA; Biological Systems & Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
  • Keasling JD; Joint BioEnergy Institute, 5885 Hollis Street, Emeryville, CA, USA; Biological Systems & Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA; California Institute of Quantitative Biosciences (QB3), University of California, Berkeley, CA, USA; Department of Chemical &am
  • Lee TS; Joint BioEnergy Institute, 5885 Hollis Street, Emeryville, CA, USA; Biological Systems & Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA. Electronic address: tslee@lbl.gov.
Metab Eng ; 84: 69-82, 2024 Jul.
Article in En | MEDLINE | ID: mdl-38839037
ABSTRACT
Sunscreen has been used for thousands of years to protect skin from ultraviolet radiation. However, the use of modern commercial sunscreen containing oxybenzone, ZnO, and TiO2 has raised concerns due to their negative effects on human health and the environment. In this study, we aim to establish an efficient microbial platform for production of shinorine, a UV light absorbing compound with anti-aging properties. First, we methodically selected an appropriate host for shinorine production by analyzing central carbon flux distribution data from prior studies alongside predictions from genome-scale metabolic models (GEMs). We enhanced shinorine productivity through CRISPRi-mediated downregulation and utilized shotgun proteomics to pinpoint potential competing pathways. Simultaneously, we improved the shinorine biosynthetic pathway by refining its design, optimizing promoter usage, and altering the strength of ribosome binding sites. Finally, we conducted amino acid feeding experiments under various conditions to identify the key limiting factors in shinorine production. The study combines meta-analysis of 13C-metabolic flux analysis, GEMs, synthetic biology, CRISPRi-mediated gene downregulation, and omics analysis to improve shinorine production, demonstrating the potential of Pseudomonas putida KT2440 as platform for shinorine production.
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Full text: 1 Database: MEDLINE Main subject: Sunscreening Agents / Pseudomonas putida / Metabolic Engineering Language: En Journal: Metab Eng Journal subject: ENGENHARIA BIOMEDICA / METABOLISMO Year: 2024 Type: Article
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Full text: 1 Database: MEDLINE Main subject: Sunscreening Agents / Pseudomonas putida / Metabolic Engineering Language: En Journal: Metab Eng Journal subject: ENGENHARIA BIOMEDICA / METABOLISMO Year: 2024 Type: Article