Respiratory Viruses Co-detection on a Multiplex RT-PCR Panel: a Comparative Clinical Study.
Clin Lab
; 70(7)2024 Jul 01.
Article
in En
| MEDLINE
| ID: mdl-38965948
ABSTRACT
BACKGROUND:
The FilmArray Respiratory Panel RP 2.1 plus (FilmArray RP) is a point-of-care syndromic panel for respiratory pathogens. Although highly valuable in the clinical settings, the co-detection of pathogens in FilmArray RP may confound result interpretation.METHODS:
Nasopharyngeal swab specimens collected from patients with respiratory symptoms were analyzed by comparing co-detection results from FilmArray RP with those of Allplex Respiratory Panels (Allplex RP Power-Chek for SARS-CoV-2).RESULTS:
Out of 765 FilmArray RP tests, 143 (18.7%) showed co-detections (two 122 (85.3%), three 18 (12.6%), four 2 (1.4%), and five viruses 1 (0.7%). The most frequent co-detection was human rhinovirus/enterovirus (HRV/HEV) with respiratory syncytial virus (RSV) (22.3%, 32/143). The overall discordance rate between Film-Array RP and other tests was 32.9%. Notably, discordance in detecting adenovirus (AdV) was significant, with cases detected by FilmArray often not appearing in Allplex RP.CONCLUSIONS:
Discordances were varied by virus combination. It is advisable to perform additional confirmatory testing based on clinical relevance.
Full text:
1
Database:
MEDLINE
Main subject:
Respiratory Tract Infections
/
Coinfection
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Multiplex Polymerase Chain Reaction
Limits:
Adolescent
/
Adult
/
Aged
/
Child
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Child, preschool
/
Female
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Humans
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Infant
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Male
/
Middle aged
Language:
En
Journal:
Clin Lab
Journal subject:
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Year:
2024
Type:
Article