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Generation of a homozygous (MCRIi031-A-3) WT1 knockout human iPSC line.
Pachernegg, Svenja; Robevska, Gorjana; Ferreira, Lucas G A; van den Bergen, Jocelyn A; Vlahos, Katerina; Howden, Sara E; Sinclair, Andrew H; Ayers, Katie L.
Affiliation
  • Pachernegg S; Reproductive Development, Murdoch Children's Research Institute, Melbourne, Australia; Department of Paediatrics, The University of Melbourne, Melbourne, Australia.
  • Robevska G; Reproductive Development, Murdoch Children's Research Institute, Melbourne, Australia.
  • Ferreira LGA; Reproductive Development, Murdoch Children's Research Institute, Melbourne, Australia; Laboratory of Molecular and Translational Endocrinology, Universidade Federal de Sao Paulo, Sao Paulo, Brazil.
  • van den Bergen JA; Reproductive Development, Murdoch Children's Research Institute, Melbourne, Australia.
  • Vlahos K; iPSC Derivation and Gene Editing Facility, Murdoch Children's Research Institute, Melbourne, Australia.
  • Howden SE; Department of Paediatrics, The University of Melbourne, Melbourne, Australia; iPSC Derivation and Gene Editing Facility, Murdoch Children's Research Institute, Melbourne, Australia; Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Murdoch Children's Research Institute, Melbourne, Austr
  • Sinclair AH; Reproductive Development, Murdoch Children's Research Institute, Melbourne, Australia; Department of Paediatrics, The University of Melbourne, Melbourne, Australia.
  • Ayers KL; Reproductive Development, Murdoch Children's Research Institute, Melbourne, Australia; Department of Paediatrics, The University of Melbourne, Melbourne, Australia. Electronic address: katie.ayers@mcri.edu.au.
Stem Cell Res ; 79: 103494, 2024 Sep.
Article in En | MEDLINE | ID: mdl-39003885
ABSTRACT
The transcription factor WT1 plays a critical role in several embryonic developmental processes such as gonadogenesis, nephrogenesis, and cardiac development. We generated a homozygous (MCRIi031-A-3) WT1 knockout induced pluripotent stem cell (iPSC) line from human fibroblasts using a one-step protocol for CRISPR/Cas9 gene-editing and episomal-based reprogramming. The cells exhibit a normal karyotype and morphology, express pluripotency markers, and have the capacity to differentiate into the three embryonic germ layers. These cell lines will allow us to further explore the role of WT1 in critical developmental processes.
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Full text: 1 Database: MEDLINE Main subject: WT1 Proteins / Induced Pluripotent Stem Cells / Homozygote Limits: Humans Language: En Journal: Stem Cell Res Year: 2024 Type: Article Affiliation country: Australia
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Full text: 1 Database: MEDLINE Main subject: WT1 Proteins / Induced Pluripotent Stem Cells / Homozygote Limits: Humans Language: En Journal: Stem Cell Res Year: 2024 Type: Article Affiliation country: Australia