Transposon Tn3-mediated replicon fusion.
Gene
; 19(3): 251-7, 1982 Oct.
Article
in En
| MEDLINE
| ID: mdl-6295878
ABSTRACT
To investigate inter-replicon transposition of Tn3, we used the cosmid-phage lambda packaging system coupled with density gradient fractionation and isolated recombinant molecules of different sizes. Cosmids derived from ampicillin-resistance-transducing phage were classified into four groups (1) cosmid-Tn3 donor cointegrates considered as Tn3 transposition intermediates, (2) similar cointegrates carrying deletions of one copy of Tn3 and of adjacent cosmid DNA sequences, (3) cosmids carrying a single Tn3 insertion, and (4) cosmids carrying two independent Tn3 insertions. Genetic and biochemical studies indicated that cosmids isolated from ampicillin-resistance transductants were derived from the authentic cosmid-Tn3 donor cointegrate intermediates.
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Database:
MEDLINE
Main subject:
Plasmids
/
DNA Transposable Elements
/
Bacteriophage lambda
/
Escherichia coli
Language:
En
Journal:
Gene
Year:
1982
Type:
Article