Pressure reversal of the action of octanol on postsynaptic membranes from Torpedo.
Br J Pharmacol
; 83(1): 305-11, 1984 Sep.
Article
in En
| MEDLINE
| ID: mdl-6487895
ABSTRACT
Octanol increases the binding of [3H]-acetylcholine to the desensitized state of the nicotinic receptor in postsynaptic membranes prepared from Torpedo californica. This increase in binding results from an increase in the affinity of [3H]-acetylcholine for its receptor without any change in the number of sites or the shape of the acetylcholine binding curve. High pressures of helium (300 atm) decrease [3H]-acetylcholine binding by a mechanism that changes only the affinity of acetylcholine binding. Helium pressure reverses the effect of octanol on the affinity of [3H]-acetylcholine for its receptor. This pressure reversal of the action of octanol at a postsynaptic membrane is consistent either with pressure counteracting an octanol-induced membrane expansion or with independent mechanisms for the actions of octanol and pressure. The data do not conform with a mechanism in which pressure displaces octanol from a binding site on the receptor protein.
Full text:
1
Database:
MEDLINE
Main subject:
Pressure
/
Synaptic Membranes
/
Receptors, Cholinergic
/
Octanols
/
Anesthetics
Limits:
Animals
Language:
En
Journal:
Br J Pharmacol
Year:
1984
Type:
Article