In vivo priming of two distinct antitumor effector populations: the role of MHC class I expression.
J Exp Med
; 179(4): 1215-24, 1994 Apr 01.
Article
in En
| MEDLINE
| ID: mdl-7908321
ABSTRACT
Downregulation of major histocompatibility complex (MHC) class I expression is an important mechanism by which tumors evade classical T cell-dependent immune responses. Therefore, a system was designed to evaluate parameters for active immunization against MHC class I- tumors. Mice were capable of rejecting a MHC class I- tumor challenge after immunization with an irradiated granulocyte/macrophage colony-stimulating factor (GM-CSF) transduced MHC class I- tumor vaccine. This response was critically dependent on CD4+ T cells and natural killer (NK) cells, but minimally on CD8+ T cells. A strong protective response against MHC class I+ variants of the tumor could be elicited when mice were immunized with irradiated MHC class I+ GM-CSF-secreting tumor cells. This response required CD4+ and CD8+ T cells, and in addition, elimination of NK cells resulted in outgrowth of tumors that had lost expression of at least one MHC class I gene. Finally, class I MHC expression on the vaccinating cells inhibited the response generated against a MHC class I- tumor challenge. These results demonstrate that the host is capable of being immunized against a tumor that has lost MHC class I expression and reveal conditions under which distinct effector cells play a role in the systemic antitumor immune response.
Full text:
1
Database:
MEDLINE
Main subject:
T-Lymphocytes
/
Histocompatibility Antigens Class I
/
Neoplasms, Experimental
Limits:
Animals
Language:
En
Journal:
J Exp Med
Year:
1994
Type:
Article