Advanced glycation end products up-regulate gene expression found in diabetic glomerular disease.
Proc Natl Acad Sci U S A
; 91(20): 9436-40, 1994 Sep 27.
Article
in En
| MEDLINE
| ID: mdl-7937785
ABSTRACT
Several lines of evidence suggest that the excessive accumulation of extracellular matrix in the glomeruli of diabetic kidneys may be due to reactive intermediates forming between glucose and matrix proteins called advanced glycation end products (AGEs). Normal mice received AGE-modified mouse serum albumin i.p. for 4 weeks, and glomerular extracellular matrix, growth factor mRNA levels, and morphology were examined. We found that AGE induced an increase in glomerular extracellular matrix alpha 1(IV) collagen, laminin B1, and transforming growth factor beta 1 mRNA levels, as measured by competitive PCR, as well as glomerular hypertrophy. The AGE response was specific because the coadministration of an AGE inhibitor, aminoguanidine, reduced all these changes. We conclude that AGEs affected expression of genes implicated in diabetic kidney disease and may play a major role in nephropathy.
Full text:
1
Database:
MEDLINE
Main subject:
Serum Albumin, Bovine
/
Gene Expression Regulation
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Glycation End Products, Advanced
/
Diabetic Nephropathies
/
Kidney
/
Kidney Glomerulus
Limits:
Animals
Language:
En
Journal:
Proc Natl Acad Sci U S A
Year:
1994
Type:
Article