Similarities between initiation of V(D)J recombination and retroviral integration.
Science
; 271(5255): 1592-4, 1996 Mar 15.
Article
in En
| MEDLINE
| ID: mdl-8599117
ABSTRACT
In the first step of V(D)J recombination, the RAG1 and RAG2 proteins cleave DNA between a signal sequence and the adjacent coding sequence, generating a blunt signal end and a coding end with a closed hairpin structure. These hairpins are intermediates leading to the formation of assembled antigen receptor genes. It is shown here that the hairpins are formed by a chemical mechanism of direct trans-esterification, very similar to the early steps of transpositional recombination and retroviral integration. A minor variation in the reaction is sufficient to divert the process from transposition to hairpin formation.
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Database:
MEDLINE
Main subject:
Recombination, Genetic
/
Gene Rearrangement
/
Gene Rearrangement, T-Lymphocyte
/
HIV
/
Virus Integration
/
Homeodomain Proteins
/
DNA-Binding Proteins
Language:
En
Journal:
Science
Year:
1996
Type:
Article
Affiliation country:
United States