Multicolor spectral karyotyping of human chromosomes.

Schröck, E; du Manoir, S; Veldman, T; Schoell, B; Wienberg, J; Ferguson-Smith, M A; Ning, Y; Ledbetter, D H; Bar-Am, I; Soenksen, D; Garini, Y; Ried, T

Schröck, E; du Manoir, S; Veldman, T; Schoell, B; Wienberg, J; Ferguson-Smith, M A; Ning, Y; Ledbetter, D H; Bar-Am, I; Soenksen, D; Garini, Y; Ried, T.

Affiliation

Schröck E; Diagnostic Development Branch, National Center for Human Genome Research, National Institutes of Health, Bethesda, MD 20892-4470, USA.

Science ; 273(5274): 494-7, 1996 Jul 26.

Article in En | MEDLINE | ID: mdl-8662537

ABSTRACT

The simultaneous and unequivocal discernment of all human chromosomes in different colors would be of significant clinical and biologic importance. Whole-genome scanning by spectral karyotyping allowed instantaneous visualization of defined emission spectra for each human chromosome after fluorescence in situ hybridization. By means of computer separation (classification) of spectra, spectrally overlapping chromosome-specific DNA probes could be resolved, and all human chromosomes were simultaneously identified.

Subject(s)

Chromosomes, Human/ultrastructure; In Situ Hybridization, Fluorescence; Karyotyping/methods; Animals; Breast Neoplasms/genetics; Chromosome Aberrations; DNA Probes; Fluorescent Dyes; Fourier Analysis; Humans; Hylobates/genetics; Image Processing, Computer-Assisted; Interferometry; Spectrum Analysis; Translocation, Genetic; Tumor Cells, Cultured

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Database: MEDLINE Main subject: Chromosomes, Human / In Situ Hybridization, Fluorescence / Karyotyping Limits: Animals / Humans Language: En Journal: Science Year: 1996 Type: Article Affiliation country: United States

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